Regulatory

Part:BBa_K105024:Design

Designed by: Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-26)


Gal4 - operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick contains the consensus sequence plus 5 spacer nucleotides on each side. The spacer is directly followed by the restriction site without a base in between. So when you construct multiple operator sites from this basic part you will have 16 bp between two consensus sequences.



Source

from oligos

References